For each product to be tested according to ASTM E2149-13a, three sterile containers are needed; one containing only the bacterial suspension, one containing the treated article in the bacterial suspension and another flask containing an untreated article, or control, also in the bacterial suspension.
For the ASTM E2149-13A method, Escherichia coli ATCC 25922 is specified as the standard organism. Nevertheless, additional organisms can be requested, as well as modifications from the standard 1-hour contact time to 24 hours, or other time periods as required.
Credibility of result
ASTM E2149-13a is a reproducible method that also allows experimental flexibility to test products of several shapes and sizes, generally consisting of treated fabrics. Routine quality controls on media are also carried out to maintain efficacy and accuracy of results.